This page contains data files associated with Ni, Bruce et al, (2009, Genes & Development).

• processed data. The data processed as detailed in Materials and Method of the paper. Files with names in the form of TF_30min.clean.null_norm.sig.sgr are processed signal files for transcription factor TF at 30min in the sgr format (chr, position, signal), suitable for viewing with IGB. Files ending with the extension .prob.sgr contain data for the probability that the red signal from all the probes in a band-width of 375 nucleotides is greater than the green signal due to chance. Files with names like TF_30min.clean.null_norm.hits.q01.out list the location of the signal peaks ("hits"), their average signal, and the names of the flanking ORFs on the W and C strands, and whether the hit is within an ORF. The hyperlink for each peak links to the ORF map at the yeastgenome.org site.
• qPCR data. Contains the data necessary to normalize the time points for Sko1 and Yap4 binding. For logistical reasons each time point of the ChIP-chip assay was done on a separate day. In order to ensure that the datasets are normalized relative to each other, a smaller scale experiment was done, all in one day, by performing ChIP qPCR on a small number of chromosomal sites. The binding values determined by qPCR was plotted for that determined by ChIP chip so as to generate a calibration curve for each ChIP chip assay. The ChIP chip data for each time point was multiplied by a correction factor that equalized the calibration curves.
• Expression data. Contains expression data for wt (t=7.5, 15, 22.5, 30, 45 and 60 min post salt induction), yap4-KO (t=0, 15, 30 and 45 min), yap6-KO (t=0,15,30 and 45min) and yap4-KO/yap6-KO (t=0, 15, 30 and 45 min) . Each data file is for a two channel hybridization experiment, where channel 2 (532) contains cDNA from wt_0min.