Privacy-aware Binary Alignment Mapping (pBAM)

• This is a manipulation on Binary Alignment Files (BAM).


• Works with the majority of trhe functions of SAMtools (exclusions are due to removal of SEQ, CIGAR, and QUAL fields), and other tools that work on BAM files.


• Works well with many functional genomics pipelines, including STAR signal track (STAR), RSEM gene expression and quantification (RSEM), and MACS2 (MACS2) for ChIP-seq peak calling.


• The original BAM does not need to be stored. Rather, a smaller file called .diff can be safely stored for sensitive information in the BAM file.


• Any BAM file can be converted to pBAM using these codes.


• pBAM files can be combined with the appropriate .diff file and reference genome to re-create the BAM file using these codes.


• For specifications, click here.