-40% -20% 0% 20% 40% 60% 80% N  C  S  L  F  Q  I   D  Y  H M W P  E  T  R  K  V  G  A Amino acid D (aa, [ w_mRNA, G_mRNA], [1, G_mRNA]) D (aa, [ w_Prot, G_mRNA], [1, G_mRNA]) D (aa, [ w_lacZ, G_lacZ], [1, G_mRNA]) a -20% -10% 0% 10% 20% 30% 40% L  W M Q  N  A  F  E  P  R  K   I   H  D  T  Y  S  G  V  C Amino Acid D (aa, [ w_mRNA, G_mRNA], [1, G_mRNA]) D (aa, [ w_Prot, G_Prot], [1, G_Prot]) D (aa, [ w_mRNA, G_Prot], [1, G_Prot ]) D (aa, [ Prot w , G_Prot], [w_mRNA, G_Prot]) b 41.7 43.2 49.1 37.2 52.3 +12% -4% -15% 108.4 108.7 109.7 110.7 112.1 -2.1% -1.3% -3.3% -0.3% -1.2% -0.9% Molecular weight per ORF [kDa] Molecular weight per amino acid [Da] -20% -12% -29% c Greenbaum et al - Figure 3